Core E Viral Pathogenesis & Persistence
Core E aims to support applied research toward an HIV cure by providing accurate quantification of HIV Reservoirs; primary cell models of latent HIV infection; simplified analysis of HIV integration sites; and analysis of individual proviral transcription. Core E will also support basic research into HIV biology, transmission, and pathogenesis with HIV single genome sequencing (SGS) and high-resolution visualization of HIV transcription. Applied research to enhance HIV prevention and treatment efforts through quantification of drug-resistant variant frequency for current and new antiretroviral agents in development is also supported by Core E.
See photos of our Core E lab here.
OUR SERVICES INCLUDE (but are not limited to)
- Lentivirus and Lentiviral Vector Production
- Donor derived virus production
- Access to the shRNA library DECIPHER project
- Nanoscale flow cytometry (NFC) training and expertise
HIV Quantification and Characterization
- Automated Plasma Single Copy Assay (autoSCA)
- Automated HIV-1 DNA and Cell-Associated HIV-1 RNA
- Quantitative or Fully Autologous Viral Outgrowth Assay
- Cell-associated DNA and RNA Single Genome Sequencing (CARD-SGS)
- Envelope detection by induced transcription-based sequencing (EDITS)
- Next Generation Sequencing (NGS) including UMI-NGS
- Viral and proviral sequencing, integration site analyses, and bioinformatics
- Phenotypic assessment of donor-derived/mutant sequence using reporter cell- lines
- Polarized Th17 and microglial models
- Human Lymphoid Aggregate Culture (HLAC)
- Polarized Explant Models
- DeltaVision Deconvolution Microscope
- Imaris Bitplane software suite
Interested in utilizing Core E or learning more about how we might be able help with you HIV-1 viral pathogenesis and pathology studies? Please contact either Director listed above or Core E Research manager Kerri Penrose.
Ready to start a project request? Project requests can be generated directly through iLAB.
CWRU iLAB : https://cwru.corefacilities.org/landing/76
UPitt iLAB: https://upmc.corefacilities.org/account/login